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murine astrocyte culture  (ATCC)


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    ATCC murine astrocyte culture
    Murine Astrocyte Culture, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+astrocyte+culture/C8-D1A/pmc13026089-37-5-10
    Average 96 stars, based on 326 article reviews
    murine astrocyte culture - by Bioz Stars, 2026-08
    96/100 stars

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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice <t>cultures</t> showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) <t>Murine</t> cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced <t>astrocytes.</t>
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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice <t>cultures</t> showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) <t>Murine</t> cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced <t>astrocytes.</t>
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    Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice cultures showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) Murine cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced astrocytes.

    Journal: Cells

    Article Title: Targeting Astrocytic Connexin 43 Mitigates Glutamate-Driven Motor Neuron Stress in Late-Onset Spinal Muscular Atrophy

    doi: 10.3390/cells14231852

    Figure Lengend Snippet: Glutamate assay showed increased expression in SMA, reversible by inhibiting Cx43. ( A ) Slice cultures showed higher glutamate levels in SMA compared to WT (*** p < 0.001). Gap27 treatment (200 µM) in SMA resulted in a decrease in glutamate levels (*** p < 0.001) to the level of WT ( p = 0.1). ( B ) Murine cell cultures also showed higher glutamate levels when SMN was knocked down (*** p < 0.001), with the levels decreasing with 200 µM Gap27 (*** p < 0.001) to the level of WT ( p = 0.85). ( C ) Similarly, hiAstrocytes showed increased glutamate levels when SMN was knocked down (*** p < 0.001). This significantly decreased after treatment with 200 µM Gap27 ( p < 0.001) to the level of the control ( p = 0.92). Analysis was conducted using unpaired Student’s t -tests. Abbreviations: SMA, spinal muscular atrophy; SMN, survival of motor neuron; WT, wild-type. hiAstrocytes, human induced astrocytes.

    Article Snippet: A genetic knockdown was achieved by transfecting WT murine astrocyte cultures with mouse-specific SMN1 -siRNA (#SR408287, OriGene, Rockville, MD, USA) to induce SMN deficiency.

    Techniques: Glutamate Assay, Expressing, Control